{"id":2429,"date":"2026-02-07T16:14:42","date_gmt":"2026-02-07T16:14:42","guid":{"rendered":"https:\/\/cbds.montes.upm.es\/?p=2429"},"modified":"2026-02-07T16:18:09","modified_gmt":"2026-02-07T16:18:09","slug":"overexpression-of-ginkbilobin-2-homologous-domain-gene-to-enhance-the-tolerance-to-phytophthora-cinnamomi-in-plants-of-european-chestnut","status":"publish","type":"post","link":"https:\/\/cbds.montes.upm.es\/?p=2429","title":{"rendered":"Overexpression of ginkbilobin-2 homologous domain gene to enhance the tolerance to Phytophthora cinnamomi in plants of European chestnut"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2429\" class=\"elementor elementor-2429\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-72f3beba elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"72f3beba\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-2547a241\" data-id=\"2547a241\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-24263cee elementor-widget elementor-widget-text-editor\" data-id=\"24263cee\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Autores: <\/strong>Susana Serrazina, M\u00aa Teresa Mart\u00ednez, Silvia Valladares, Luc\u00eda Del Castillo-Gonz\u00e1lez, Marcelo Francisco, Marta Berrocal-Lobo, Eduardo Pi\u00f1as, Pablo Pi\u00f1eiro, Rui Malh\u00f3, Rita Louren\u00e7o Costa &amp; Elena Corredoira<\/p><p><strong>Publicaci\u00f3n:<\/strong> BMC Genomics 2026.<\/p><p><strong>Enlace DOI: <a href=\"https:\/\/doi.org\/10.1186\/s12864-025-12485-x\">https:\/\/doi.org\/10.1186\/s12864-025-12485-x<\/a><\/strong><\/p><p><strong>Resumen:<\/strong>\u00a0<\/p><p><i>Castanea sativa<\/i>, a species of high ecological and economic relevance in Europe, faces severe threats from root rot caused by <i>Phytophthora cinnamomi<\/i>. To explore genetic strategies for enhancing disease tolerance, we investigated the functional role of a chestnut gene homologous to <i>Ginkgo biloba<\/i>\u2019s ginkbilobin-2 (<i>Cast_Gnk2-like<\/i>), known for its antifungal properties. Using <i>Agrobacterium tumefaciens<\/i>-mediated transformation, the <i>Cast_Gnk2-like<\/i> gene was introduced into somatic embryos from two embryogenic chestnut lines. Transformation efficiency was genotype-dependent, and varied from 14.2% to 2.5%. Twelve independent transgenic lines were confirmed by PCR, and each was estimated to carry a single copy of the transgene. Gene expression analysis revealed significant <i>Cast_Gnk2-like<\/i> transcript levels in two transgenic lines. Following cold storage and germination treatment, viable transgenic plants were regenerated. Disease tolerance assays demonstrated that <i>Cast_Gnk2-like<\/i> overexpression significantly reduced root necrosis and symptom severity, indicating enhanced tolerance to <i>P. cinnamomi<\/i>. These findings highlight the potential of targeted gene overexpression to improve disease resilience in chestnut through genetic engineering.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Autores: Susana Serrazina, M\u00aa Teresa Mart\u00ednez, Silvia Valladares, Luc\u00eda Del Castillo-Gonz\u00e1lez, Marcelo Francisco, Marta Berrocal-Lobo, Eduardo Pi\u00f1as, Pablo Pi\u00f1eiro, Rui Malh\u00f3, Rita Louren\u00e7o Costa &amp; Elena Corredoira Publicaci\u00f3n: BMC Genomics 2026. Enlace DOI: https:\/\/doi.org\/10.1186\/s12864-025-12485-x Resumen:\u00a0 Castanea sativa, a species of high ecological and economic relevance in Europe, faces severe threats from root rot caused by [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":2431,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[9],"tags":[],"class_list":["post-2429","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articulos"],"_links":{"self":[{"href":"https:\/\/cbds.montes.upm.es\/index.php?rest_route=\/wp\/v2\/posts\/2429","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cbds.montes.upm.es\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cbds.montes.upm.es\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cbds.montes.upm.es\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/cbds.montes.upm.es\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2429"}],"version-history":[{"count":4,"href":"https:\/\/cbds.montes.upm.es\/index.php?rest_route=\/wp\/v2\/posts\/2429\/revisions"}],"predecessor-version":[{"id":2434,"href":"https:\/\/cbds.montes.upm.es\/index.php?rest_route=\/wp\/v2\/posts\/2429\/revisions\/2434"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cbds.montes.upm.es\/index.php?rest_route=\/wp\/v2\/media\/2431"}],"wp:attachment":[{"href":"https:\/\/cbds.montes.upm.es\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2429"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cbds.montes.upm.es\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2429"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cbds.montes.upm.es\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2429"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}